Trypsin edta phenol red,penicillin-streptomycin,trypan blue solution,advanced dmem,rpmi 1640 medium,fetal bovine serum,dimethyl sulfoxide sterile,10x pbs,syringe-driven filters,sodium chloride,50x tae buffer,tris base,magnesium sulphate heptahydrate,tris hydrochloride,sm buffer,taq dna polymerase 2x master mix,sterile pasteur pipette,polyethylene glycol 8000,polyethylene glycol 10000,self standing centrifuge tubes,multidish 6 well cell culture plates,multidish 12 well cell culture plates,4 way tube rack,conical centrifuge tube rack,mr. cell freezing container,1 kb dna ladder,5x sucrose gel loading dye,nitrile gloves,kimwipes